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KMID : 0352519950320030339
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Korea Univercity Medical Journal
1995 Volume.32 No. 3 p.339 ~ p.355
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An Immunochemical Study of Hepatic Tissue Using Labeled Monoclonal Anti-γ Glutamyltransferase
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Ham Soo-Youn
Choe Jae-Gol
Lee Min-Jai
Chung Kyoo-Byung
Kim Meyoung-Kon
Ryu Chong-Kon
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Abstract
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This study was designed to investigate whether radioisotope labeled tissue-specific monoclonal anti-γ lutamyltransferase (GGT) antibodies can be used for the scintigraphic localization of abnormal hepatic tissue of rat. Three monoclonal anti-GGT antibodies (GGT MAb-1, 5, 6) were biochemically purified from the ascites of BALB/c mice which were injected hybridomas secreting monoclonal antibody to a 7.17 mg of immunoglobulin per ml of protein and the mean mmunoglobulin amount was 4.98 mg. Then I-131 was incorporated to GGT MAbs by lodogen method with an average labeling efficiency of 77.4% (GGT MAb), and 86.8% (GGT PAb) and in case of Tc-99m by sodium dodesylsulfate, they were 57.7% (GGT MAb), and 77.6% (GGT PAb) respectively. In vitro assay revealed that there was a significant correlation between the result of immunohistochemistry and that of tissue autoradiography according to the type of tissue and monoclonal antibodies in normal heaptic tissue, preneoplastic liver and normal kidney. Modified GGT MAb-1 was specific for the hepatic tissue of preneoplastic liver, GGT MAb-5 for the normal liver, and GGT MAb-6 for the normal kidney. In vivo assay revealed selective localization of radioactivity over tissue specific GGT distributed organs of Wister rat by immunoscintigraphy and tissue γ-counting.
In conclusion, radioisotope labeled anti-GGT antibodies might be used for both in vitro and in vivo assays and could be used for the localization of musine hepatic disorders by immunoscintigraphy.
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